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EPTOMICS代理Atg12 （ATG12） antibody Eptomics公司抗體供應商

Rabbit Monoclonal Antibody

Description

Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. Atg12 is the human homolog of a yeast protein involved in autophagy （1）. It is essential for Atg12 conjugation, and the ATG12-ATG5 conjugate is necessary to form autophagic vesicles （2）. The E3-like activity of the Atg12-Atg5 conjugate potentiates the formation of Atg8-PE; making the autophagic pathway analogous to ubiquitin-like pathways where an E3 conjugation ligase is required （3）.

Recommended Applications

WB, IHC, ICC, IP, FC

Applications and Recommended Dilution Factors

WB: 1:1,000-10,000
IHC: 1:50-100
ICC: 1:100-250
IP: 1:10-100
FC: 1:10-100
 

Species Reactivity *

Human

*Species cross-reactivity is based on WB analysis

Products Data

Western blot analysis on （A） THP-1, （B） HepG2, （C） Raji, and （D） fetal kidney lysates using anti-Atg12 RabMAb （cat. #3473-1）.	Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Atg12 RabMAb （cat. #3473-1）.	Western blot analysis on （A） Atg12 transfected 293T and （B） 293T lysates using anti-Atg12 RabMAb （cat. #3473-1）.

Specificity

A synthetic peptide corresponding to residues in human Atg12 was used as an immunogen.

Storage Condition and Buffer

Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.

Alternative Names

ATG12 antibody, APG12 antibody, APG12L antibody, FBR93 antibody, HAPG12 antibody, Ubiquitin-like protein ATG12 antibody, Autophagy-related protein 12 antibody

Description References

1. Entrez Gene: gene-centered information at NCBI. Nucleic Acids Res. 2005 Jan 1;33:D54-8.
2. Phillips AR et al. Genetics. 2008 Mar;178（3）:1339-53.
3. Williams RA et al. Autophagy. 2009 Feb;5（2）:159-72.

EPTOMICS代理Atg12 （ATG12） antibody Eptomics公司抗體供應商 

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